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Total RNA extraction and quantitative real-time PCR (qRT-PCR) Total RNA was extracted from liver tissues or cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA)

Do not flush or pour supplements down the sink, as this can contribute to water pollution, with substances potentially contaminating sources since they may not be fully filtered by treatment plants

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Vines were the subject to various levels of N status, while vine water status and vigor were controlled

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