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To monitor and modulate integration and circuit engagement of hPS cell-derived DA neurons, a clozapine- N -oxide (CNO)-responsive designer receptors exclusively activated by designer drug (DREADDs) cassette was inserted via CRISPR knock-in, allowing the chemogenetic activation or inhibition of grafted cells 92

A more recent study also used ultrasensitive single-molecule arrays to detect brain injury biomarkers in urine from patients with IS and intracerebral hemorrhage, and found that higher urine GFAP (and to a lesser extent urine NfL and t-tau) levels in patients correlated with injury burden and in-hospital death or death by 3 months (77)

Kade IJ, Balogun BD, Rocha JB

In summary, advancing the field of human neuroprotection demands a multidimensional recalibration, spanning conceptual clarity, more representative preclinical models, validated biomarkers, and refined clinical endpoints