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The absorbency was read at 580 nm

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After adjusting the protein concentration using RIPA buffer, an equal amount of total protein (20 g) was resolved on 5% acrylamide gels (Amresco), and proteins were electrophoretically separated on SDS gels (1015%, Sigma) at a constant voltage of 120 V for 20 min ( n = 6 per group)

Downstream of ROS accumulation, COX-2 and iNOS are key pro-inflammatory enzymes that amplify oxidative and immune-mediated damage

Disodium EDTA (Chelation) IV Drip Disodium EDTA IV chelation (pronounced kee-LAY-shun) therapy is a provider-directed treatment primarily used to support vascular health and heavy metal detoxification