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The enzyme was dialyzed by 20 kDa cut-off membrane (Cat#0.54600051) against polyethylene glycol 6000 till minimum volume, precipitated by acetone (1:1 v/v), the pellets were dissolved in PP buffer (pH 6.5, 10 mM)

S.A., M.E., J.S., J.R., and A.L

3.8 Correlations among gut microbiota, metabolites and immunity To explore the interplay between gut microbiota, microbial metabolites, and the host immune system, we conducted Spearman correlation analysis and visualized the results as a heatmap (Figure 8)

First described in the 1990s by researchers at the University of Zagreb, it has been studied across a broad range of preclinical models for regenerative and cytoprotective properties

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