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The main goal of T1D treatment is to maintain blood glucose at a normal range to reduce severe diabetes-associated complications

Treatment: High-dose folinic acid (leucovorin): Bypasses FR by using alternative transporters (e.g., reduced folate carrier, RFC)

Following this, the bacterial culture was pelleted by centrifugation at 1,000 g for 5 min at room temperature and then resuspended in the medium designated for further experiments