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The blue formazan salt formed by cellular mitochondria was extracted with isopropanol, and the optical density of the extracted formazan was determined using a spectrophotometer (SpectraMax M5, USA) at 570 nm

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The results showed that peptone, KH 2 PO 4 and glutamate were the three most significant factors affecting the GSH production of S

e,h , Observed adducts and fragments in negative ( e ) and positive ( h ) ion ESI-MS, their calculated monoisotopic masses, as well as the expected and observed number of 18 O incorporations