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Methods Cell culture RAW 264.7 cells (ATCC) were cultured in RPMI 1640 with 25 mM HEPES, 1% penicillin/streptomycin and 10% FBS (dialyzed FBS was used for the metabolite extraction experiments) at 37 C and 5% CO 2

However, efficacy may vary and multiple sessions are needed

A Kinetic Safety Gate Controlling the Delivery of Unnatural Amino Acids to the Ribosome

Agarwal R, Burley SK, Swaminathan S

Lancet 397 , 194 (2021)

Thus, changes in these biomarkers do not accurately assess real-time brain function since the underlying and established deficits in neuronal functioning are unaffected by their absence