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By fusing these promoter sequences to lacZ gene, we successfully certified that 8 out of 22 candidates exhibiting high -galactosidase activities that are 35 times higher than the control promoter P hsp60 (Figure 10)

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Given that rutin is notably marked by its therapeutically essential antioxidant polyphenols 106,107

Additionally, PAB increased the production of H2O2 and lipid peroxides by causing intracellular GSH depletion via the p53-mediated xCT pathway

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These effects are mediated through inhibition of the NF-B/NLRP3/caspase-1 and Bax/Bcl-2/caspase-3 pathways, alongside activation of Nrf2/HO-1, p-AMPK, and -Klotho signaling (Khallaf et al