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Following this, the samples were sequentially incubated with primary and secondary antibodies, and nuclei were stained with 4',6-diamino-2-phenylindole (DAPI) (Beyotime Biotechnology, China)

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W., Haverkos, B

In immature osteoblasts in vitro, GCs exposure was associated with cell cycle arrest in G1 phase due to down-regulation of cell cycle activators such as cyclin A, cyclin D, cyclin-dependent kinase 2 (CDK2), CDK4 and CDK6 [21,22,23], as well as activation of cell cycle inhibitors such as p53, p21 and p27 [21, 24]