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Proteins were eluted by incubation with 25 L of 100 g/ml 3xFLAG peptide (Sigma) in 0.05% RapiGest (Waters Corp) in IP buffer on an end-over-end tube rotator at 10 rpm at 4 C overnight

6b), the increase in the peak shift slowed down significantly

Additionally, hypoxia enhances the anti-inflammatory capacity of BM-MSCs, such as inhibiting IL-8 and increasing IL-1ra and GM-CSF expression (Yao et al., 2017

Nitric oxide (NO) is recognized as a mediator in a broad array of biologic systems

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When combining peptides, it is advisable to start with conservative doses of each compound and gradually adjust based on individual response and tolerance