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Applications of Synthetic Biology The synthesis of THC has been optimized through the auspices of synthetic biology techniques and microbial engineering, resulting in enhanced production efficiency and reduced costs

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Materials and Methods Cell Culture 3T3 fibroblasts were cultured in Dulbecco's modified Eagle's medium supplemented with 10% foetal calf serum (Invitrogen, San Diego, CA, USA) and antibiotics (25 U/ml penicillin, 25 g/ml streptomycin, and 0.3 g/ml amphotericin B) (Invitrogen, San Diego, CA, USA) in 5% CO 2 in air at 37C in 25 or 75 cm 2 flasks

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For example, silymarin, an antihepatotoxic agent, is a widely accepted drug for the treatment of liver diseases (6)

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Burke MJ, Fried PJ, Pascual-Leone A

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