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RPMI 1640 culture medium contained 10% FCS, 100 units/ml penicillin, 100 g/ml streptomycin, 1 mM sodium pyruvate, 1x MEM non-essential amino acids and 10 g/ml ciprofloxacin, supplemented with selection antibiotics G418 (1.5 mg/ml), hygromycin (300 g/ml, both Genaxxon), puromycin (1 g/ml, Sigma) and blasticidin (3 g/ml, PAA)

ClinicalTrials.gov NCT04184622

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A reaction mixture of H 2 O 2 solution (30%) and Tris-HCl buffer (1 M, pH7.4) was added to the cell extract, and the reaction was carried out for 2 min