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Mouse embryonic fibroblast cell line NIH/3T3 (#CRL-1658, ATCC), 293 T cells (#CRL-3216, ATCC) and normal liver cell NCTC1469 (#CCL-9.1, ATCC) were cultured in DMEM medium (#6123047, Gibco) with 10% FBS

According to the literature, NO could protect against superoxide-derived ROS and oxidative damage (Wink et al., 1995

The findings suggested an inverse relationship, where higher selenium levels were associated with a reduced risk of obesity, indicating a potential protective role of selenium against childhood obesity ( 2.2.2 The influence of selenium on diabetes mellitus Diabetes mellitus has been increasingly acknowledged as a crucial risk factor influencing the onset of HCC