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Synaptogenesis and Dendritic Spine Formation Independent of the specific HGF-binding biochemistry, Dihexa treatment of cultured rat hippocampal neurons was reported to increase dendritic spine density roughly three-fold over 5 days (approximately 41 spines per 50 um of dendrite with Dihexa versus 15 with vehicle), with newly formed spines containing normal synaptic machinery (VGLUT1, synapsin, PSD-95) and functional AMPA-receptor-mediated activity

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Elevated cyclic AMP can activate downstream signaling intermediates including protein kinase A and related phosphorylation cascades that regulate gene transcription and cellular metabolic responses

Brief temperature excursions of a few hours are unlikely to cause significant degradation

Inhibition of glutamine transport depletes glutamate and GABA neurotransmitter pools: Further evidence for metabolic compartmentation

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