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Finally, the specific productivity (qRBD) in pg/cell/day (pcd) was determined using the equation: 2.7 Small-scale purification of RBD (319-541)-His6 To purify the RBD protein from CHO-K1 cell-free culture supernatant, the supernatant was conditioned with 100 mM sodium phosphate buffer (pH 7.4) containing 1.5 M NaCl, 25 mM imidazole, and 50 M copper sulfate, if copper sulfate was not already added to the culture medium

10.1038/srep37288 46

Role of organic cation transporter 3 (SLC22A3) and its missense variants in the pharmacologic action of metformin

The first meeting took place when the children were about five months old

Sperber W., Kliniken Esslingen, Department of Neurology

[DOI] [PMC free article] [PubMed] [Google Scholar] 388.Narasimhan V, Siddique RH, Lee JO, Kumar S, Ndjamen B, Du J, Hong N, Sretavan D and Choo H, Nat