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Osteopontin silencing attenuates bleomycin-induced murine pulmonary fibrosis by regulating epithelialmesenchymal transition

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[DOI] [PMC free article] [PubMed] [Google Scholar] 39.Gomaa E.Z

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Successfully transfected cell types include: Fibroblast (3T3, COS-7) Myoblast (C2C12, L6 CRL-1458) Hepatocyte (HepG2, HuH7) Erythroleukemic cell (K562) Breast carcinoma (MCF7, Hs578T) Prostate carcinoma (LNCap) Lung carcinoma (A549, NCI-H460) Osteosarcoma (U-2 OS, Saos-2) Colon carcinoma (Caco2, SW480) Pancreatic carcinoma (PANC-1) Skin melanoma (SK-MEL-28) Transfect Common Cells Efficiently and Cost-Effectively For common cell types, Lipofectamine 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount

Finally, followed by sacrifice, the peripheral blood from the ophthalmic vein was collected, and submandibular gland tissues were rapidly collected for analysis