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This is because their stomach acid drops with age

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However, if youre vegan, vegetarian, or have absorption issues, food alone may not be enough

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8 mM of reducing agent DTT (Sigma) was then added to the samples and incubated in a thermomixer at 30C for 30 minutes, followed by the addition of 20 mM iodoacetamide (Sigma) for protein carboxylation with incubation at 30C in the dark for 30 minutes