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doi: 10.1016/j.jbc.2021.101365 70 GnanapradeepanKBasuSBarnoudTBudina-KolometsAKungCPMurphyME

The effects of normal as compared with low hematocrit values in patients with cardiac disease who are receiving hemodialysis and epoetin

This observed transcriptional shift in the SEC population aligns with established histological reports of sinusoidal rarefaction and loss of integrity in the aged marrow 27,52

Additionally, animal studies such as the one by Gurger et al

Immunoprecipitation Cells were harvested 36 h post-transfection and then lysed in radioimmunoprecipitation assay (RIPA) lysis buffer (50 mM Tris-HCl, 150 mM NaCl, 0.5% sodium deoxycholate, 1% IGEPAL, 1 mM NaF, 1 mM Na3VO4) containing a protease inhibitor cocktail and a phosphatase inhibitor cocktail (Sigma)