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Finally, folate-mediated one carbon metabolism, in which its activity is induced in LPS-activated Ms (54) also contributes to the regeneration of reduced NADPH levels, with serine and glycine as the major carbon sources of this pathway

Proteins were removed by centrifugation at 12,000 rpm at 4 C for 20 min and the supernatant was transferred to a glass vial and sealed tightly

The first incubation lasted 1 h, followed by the second incubation of 30 min

The most common side effects are nausea, diarrhea, constipation, vomiting, and temporary changes in appetite