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While this limitation exists, this shouldnt be taken as a flaw in the experimental design, since this method is suitable to support FBBD campaigns 100

The injection site was monitored for leakage, and the incision was sutured

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Subsequent purification included size exclusion chromatography using a Superdex 200 column (GE Healthcare) and further purification steps with glutathione Sepharose 4B and Ni Sepharose resins to remove residual GST and TEV protease

Cancer 2 , 157173 (2021)

Redox profiling was done for both plasma and red blood cells using NADMED methodology