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To validate such claims, bed rest studies were utilized to simulate those factors (Kehler et al

Isomalt is an equimolar mixture of two mutually diastereomeric disaccharides, each composed of two sugars: glucose and mannitol --D-glucopyranosido-16-mannitol- and also glucose and sorbitol --D-glucopyranosido-16-sorbitol-

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Sections were washed twice in TBST, followed by detection of secondary antibody using chromogen substrate buffer DAB+ (#K3468, Dako North America, Inc., Carpinteria, CA, USA) and DAB-chromogen (#K3468, Dako North America, Inc., Carpinteria, CA, USA)

For PAS staining to evaluate glycogen and basement membranes, tissue sections were oxidized with periodic acid (C0142S, Beyotime Biotechnology, China), treated with Schiffs reagent (C0142S, Beyotime Biotechnology, China), and counterstained with Hematoxylin (Bry-0001-01, Runner bio, China)