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We prepared HEK293T cells expressing cell surface-targeted LaccID, fixed the cells and overlaid them with DAB

Eur J Pharmacol 583(1):7383 di Maio R, Mastroberardino PG, Hu X et al (2013) Thiol oxidation and altered NR2B/NMDA receptor functions in in vitro and in vivo pilocarpine models: implications for epileptogenesis [J]

Alternatively, the affinity matrix can be mixed directly with the protein lysates, agitated for some amount of time, centrifuged and collected in batch selection methods

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