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Next, cDNA was synthesized by reverse transcription using the QuantiTect Reverse Transcription Kit from Qiagen according to the manufacturers instructions, and qRT-PCR was finally performed using a QuantiTect SYBR Green PCR Kit and QuantiTect Primer Assays (Qiagen) on a LightCycler 480 instrument (Roche)

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Mullane says that this sublingual liquid is ideal for vegans and contains three forms of B12 in an orange-flavoured liquid. The complex contains methylcobalamin, hydroxocobalamin and adenosylcobalamin three biologically active forms of B12