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Tag cleavage must be performed after primary purification and requires a second clean up step to remove the cleaved tag from solution

If you are planning to inject after reconstituting your HCG, you can also prep a second syringe, needle, and alcohol wipe

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Nat Neurosci 24:15081521

Notably, although only 9.93% of genomic CpGs are found in exons, nearly 40% of methylated CpGs are found in exons (averaged across treatments), suggesting CpGs in these regions are preferentially methylated