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To facilitate nucleosome remodeling, additional interactions with DNA and histone proteins are necessary (Clapier et al, 2017

De Vos KJ, Chapman AL, et al

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In turn, the pool of GC is dependent on a prior reaction with glutamate cysteine ligase, requiring glutamate and CYS (Reference Peachey, Dawson and Harper51)

To this end, we generated strains in which either Taf12 or Taf12L was expressed from a maltose-regulatable promoter ( P MAL2 ), while TFIID and SAGA specific subunits, Taf11 and Ada2 respectively, were TAP-tagged