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Merck Millipore, Burlington, MA, USA) staining was carried out according to the manufacturers instructions

ATP should then be rapidly used up and have a low measured concentration

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More ABC genes relative expression

The secondary plot for the first k obs values from the second phase yielded a rate contant at GSSG saturation around 10 s 1 for all three PfGrx variants (Fig

After that, 50 L 20% HClO 4 was added to the 450 L of pretreated tissue, blood samples, or thawed plasma samples, and the proteins were precipitated by centrifugation at 19,090 g for 5 min at 4C