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To ensure robust and statistically reliable comparisons across treatments, we focused the downstream analysis on metabolites that passed quality filters and showed reproducible detection across all biological replicates in each treatment group (CK-stress, stress + UFe, stress + FFA, and CK non-stress), with all metabolite levels normalized to the CK non-stress baseline

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A good nights sleep of 7-8 hours uninterrupted is crucial for optimal testosterone levels and robust mitochondrial health

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The increase in -catenin-positive markers indicates that CaT and CaT-ME promoted the expression of -catenin (Fig