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Monochrome images of PE and oxidized PE (oxPE) abundance localization from the tissues were generated from the MALDI-MS data using SCILS LAB 2023c Pro v11.02.14724 (Bruker Daltonics) for m / z corresponding to [M-H] of oxPE species and their non-oxidized PE precursors

Remaining worm pellet ( endo -metabolome) was transferred to a 15 mL conical tube and washed with 10 mL M9 solution, centrifuged (200 g , 22 C, 30 s), supernatant removed, and worm pellet washed twice more with M9 solution before snap freezing in liquid nitrogen

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Mixing amitriptyline with alcohol can lead to intensified side effects such as sedation and gastrointestinal problems