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248 Also, SAM has a key role in regulating liver homeostasis, and reduced levels of SAM synthesis have been detected in various chronic liver injuries, such as NAFLD

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Cryosections staining and microscopy Hematoxylin and eosin (H&E) and immunofluorescence of tissues were performed on snap-frozen tissue samples in O.C.T

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In the absence of these bases, the outcome might be minimal