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[111] The microbiological assay has been the most commonly used assay technique for foods, utilizing certain vitamin B 12 -requiring microorganisms, such as Lactobacillus delbrueckii subsp

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At the end of this step, 20 L of 200 mM DTT was added before digestion with 50 L trypsin overnight, at 37C

B12 plays an essential role in red blood cell formation helping to prevent a type of anaemia, cell metabolism, nerve function and the production of DNA - the building blocks for all your body's cells