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Cell growth assay Cells were plated at 2,500 cells per well in 96-well plates for calcein-AM staining or 7,500 cells per well in 48-well plates for cell counting

The convergence of these mechanisms is reflected in the significant alleviation of core ASD symptoms (e.g., improved social interaction and language communication, decreased repetitive/compulsive behaviors), co-morbid GI distress, sleep disorders, and a measurable reduction in systemic inflammation and standardized behavioral scores (e.g., ABC, CARS) (Kang et al., 2017

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However, the complexes induced significant DNA platination, suggesting DNA binding as their primary mechanism of action