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The Ni-NTA resin was packed into a glass column and washed with 25 column volumes of 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 0.01% (w/v) LMNG and 0.0006% (w/v) CHS, 1 M peptide, followed by elution using the same buffer supplemented with 250 mM imidazole and 3 mM CaCl 2

It isnot intended for human ingestion, medical use, veterinary treatment, or any form of in vivo application

Ensure the site is clean, then pinch or stretch the skin at the injection site

M., Jog, S., Preece, J

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The comprehensive neurodevelopmental tools such as the Bayley-III and TIMP will be administered by the medical doctors and psychologists