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Figure 2 RA is classified into two major subtypes based on the presence or absence of anti-citrullinated protein antibodies (ACPAs), detectable in around 67% of patients and serving as a key diagnostic marker (49)

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Different detection techniques can be used after bisulfite conversion, including digestion by restriction enzymes followed by PCR (Combined Bisulfite Restriction Analysis, COBRA) [71, 72], direct sequencing [73], cloning and sequencing [74], pyrosequencing [75] or mass spectrometry analysis [76]

100 Moreover, in another study, acetic acid inhibited liver fat accumulation without changing food consumption or skeletal muscle weight, and was also associated with the PPAR and AMPK pathways

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