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We adapted the isolation protocol by including a step of EpCAM magnetic activated cell sorting (MACS), which allowed us to obtain viable hepatocytes from the EpCAM-negative fraction while generating cholangiocyte organoids by culturing the EpCAM-positive cholangiocyte fraction in our reported cholangiocyte medium 4 (Fig

The presence of inflammatory mediators can induce further activation of microglia and astrocytes, which in turn release additional inflammatory factors, leading to even greater neuronal damage

2013;305:C34854

for the in vivo experiment, mice were administered BrdU (100 mg/kg) intraperitoneally on a daily basis for a period of 7 days, commencing from 8 days post-lesion (dpl) to 14 dpl

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All of these form part of the perfect muscle-building environment, and thus IGF 1 LR3 can help with this when combined with training, diet, and the correct polypharmacology