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While humoral immunity is affected to a lesser extent, antibody affinity and response is generally decreased in PEM (28)

used four different culture media to induce brain-derived neurotrophic factor (BDNF) expression in bone marrow mesenchymal stem cells isolated from humans, including serum-containing medium (MSC-medium), serum-free medium (NSC-medium), and their corresponding media supplemented with basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF)

The spheres were more homogenously distributed between 10 m and 200 m (Figure 5)

Samples were then precipitated by ice cold H 2 O/MeOH/CHCl 3 (v/v 4:4:1) washed and resuspended in 50 mM HEPES buffer (pH 7.4)

J Biol Chem 277 : 2257322580

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