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Estrogen and progesterone levels are at their lowest , and the hypothalamus responds by increasing secretion of gonadotropin releasing hormone (GnRH), which triggers pituitary production of gonadotropinsfollicle stimulating hormone (FSH) and luteinizing hormone (LH)

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The unbalanced regulation of two enzymes in the same cycle suggests that an alternative pathway exists to convert GSSG back to GSH

Since GSH binding has been shown to stabilise PV 17,23 , we next used the PaSTRy assay 24 to demonstrate that GSH also shows a modest dose-dependent stabilisation of EV-F3 (Methods, Supplementary Fig