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The band densities of the proteins of interest were quantified using the Odyssey Infrared imaging system (Li-COR) and normalized by corresponding total actin bands

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Br J Haematol 127:127139 Fung EB, Harmatz PR, Milet M, Coates TD, Thompson AA, Ranalli M, Mignaca R, Scher C, Giardina P, Robertson S, Neumayr L, Vichinsky EP, Multi-Center Iron Overload Study Group (2008) Fracture prevalence and relationship to endocrinopathy in iron overloaded patients with sickle cell disease and thalassemia