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After the desired time elapsed, 10 L of Cell Counting Kit-8 (CCK-8, Merck Life Science) was added to each well to obtain a final concentration of 0.5 mg/mL CCK-8 and plates again were incubated in 5% CO 2 at 37 C for 2 h

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Fletcher M, Ramirez ME, Sierra RA, Raber P, Thevenot P, Al-Khami AA, et al

Ruiz-Pablos believe ME/CFS, Long Covid, and post-vaccine COVID-19 syndrome are related to (if not examples of) autoimmune/inflammatory syndrome induced by adjuvants (ASIA), a syndrome for which vaccine adjuvants cause symptoms