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Lee et al., In addition to IGF-1-induced neuronal protection by modulation of the microglial inflammatory response, IGF-1 mediate a direct effect on neurons (see above), which may lead to indirect (i.e., neuron-mediated) modulation of the glial inflammatory response

At various intervals, they took MRI images of the mices discs and used a technique for imaging proteins called immunofluorescent staining to observe progress