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Western Blot Analysis Western blot analysis of the crude extracts was performed by obtaining the total protein from cyanobacteria ( A 730 = 0.3) with suspension in five volumes of cold extraction buffer containing 150 mM NaCl, 50 mM Tris-HCl (pH 7.3), 1 mM EDTA, 2% -mercaptoethanol, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride (PMSF), and an equal of volume of phenol saturated with Tris-HCl (pH 7.3)

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Wouters for the HCT116 Flp-in cell line

The initial product was obtained by heating the reaction mixture at 90 0C for15 minutes, which gave an absorption band with max at 460 nm

P., & Chanarin, I

Our previous investigation has demonstrated that SL is effective in suppressing the calcium influx in RAW264.7 cells stimulated with LPS 25