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( b and c ) Sample gating strategy for negative cells (b) and positive cells (sensor-expressing cells, c)

2.5 Sequencing data processing and species annotation The reads from each sample were spliced using Vsearch v2.15.0 in order to obtain the raw Tags data (Raw Tags)

doi: 10.1016/j.alcohol.2007.11.003 82 WirleitnerBSchroecksnadelKWinklerCSchennachHFuchsD

Cryo-EM - model building, refinement, and analysis After visual inspection of the final map, known crystal structures of Nedd4-2 domains, namely C2 (PDB: 2NSQ) and HECT (PDB: 2ONI), were fitted using the jiggle-fit tool in Coot 0.9.8 61

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