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Prior to sample loading the C18 disk was prepared by sequential treatment with methanol (20 L), acetonitrile/water/TFA (solvent B, 20 L, 80/20/0.1, v/v/v) and finally with solvent A (20 L)

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This is why a layered, personalised approach not a single peptide tends to make more clinical sense for complex post-viral presentations

It does not suppress endogenous hormone production and does not require post-cycle therapy (PCT)

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