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All groups began at 0.5mg with gradual escalation

Functional Roles of Metabolic Intermediates in Regulating the Human Mitochondrial NAD(P)+-Dependent Malic Enzyme

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metabolite classification Cross-Omics Integration Pathway mapping of shared KEGG/GO terms Genemetabolite correlation analysis (Pearson/WGCNA) Consistency testing using CCorA Data Interpretation Network reconstruction linking regulatory genes with metabolites Highlighting of key pathways and candidate biomarkers Preparation of publication-ready figures and models Quality Control Sequencing QC: base quality scores, mapping rates, read distribution Metabolomics QC: retention time stability, signal-to-noise ratio, metabolite identification levels Cross-omics QC: replicate correlation, coefficient of variation (CV), internal standard monitoring Reporting: all QC metrics are documented and included in the final report Data Delivery Clients receive a complete package including raw and processed data, bioinformatics reports, and figures