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Scientific References Berberine as a Bioactive Alkaloid: Multi-Omics Perspectives on Its Role in Obesity Management

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In this study, we tested the hypothesis that CHAC1 inhibition may preserve the intracellular glutathione in skeletal muscles and thus represent a novel strategy to treat muscle wasting in cachexia conditions

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Approximately 100 mg of tissue was then transferred to a 1.5-mL Eppendorf tube, followed by homogenization on ice using an electrical disperser (T 10 basic ULTRA-TURRAX 558 from IKA, equipped with an S10N-5G Dispersing Element) in 1 mL of 2 Sample Buffer (100 mM Tris-HCl, pH 6.8, 4% (m/v) SDS, 20% (v/v) glycerol, and 4% (v/v