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Processing of the brains After assessment of the motor performance, rats were euthanized by injection of ketamine and xylazine (80 mg/kg: 12 mg/kg, i.p.)

Plates were washed in running water, air-dried and the stain dissolved in 10% acetic acid on a shaker before absorbance at 595 nm

As the Figure 7 illustrates, the group treated with the BHE extract showed a TNF level of 8.680.33 pg.mg of protein 1 , which is less than half of the value corresponding to the control group: 19.981.61 pg.mg of protein 1

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Cell Chem Biol 30(3):321336e6