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International Patent Pending) (dOdsample 340nm /min)/(dOdcontorl 340nm /min) x Control Value = Sample Value (Activity in U/g Hb) Odsample 405nm /ODcontrol 405nm wherein dODsample is the change in optical density (per minute) for the sample, 340nm is the wavelength at which optical density is measured under kinetic mode, dODcontrol is the change in optical density (per minute) of a control measured under kinetic mode conditions at a particular wavelength (340nm), ODsample is the optical density for the sample measured once at a particular wavelength (405nm), and ODcontrol is the optical density of the control measured once at that particular wavelength (405nm), 405nm is the wavelength at which a reading of Heamoglobin contained can be taken, the control value being determined by the change in optical density (per minute) of the control measured under kinetic mode at 340nm divided by the optical density of the control at 405nm i.e

Protein-conjugated sensors were moved and immersed into wells containing pure assay buffer and equilibrated in the buffer for 10 minutes to eliminate loose, nonspecific binding proteins and establish a stable baseline

doi: 10.1038/NI805 42 KnippertzIHesseASchunderTKmpgenEBrennerMKSchulerGet al