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Next, cells in both the experimental wells and the control wells were fixed with 4% formaldehyde in PBS for 30 min and incubated without shaking at room temperature on the benchtop

To determine the specific role of GSH in tbb-6 regulation, we knocked down gamma-glutamylcysteine synthetase (-GCS), the first enzyme in GSH biosynthetic pathway

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Tregs are central inhibitory cells in the tumor microenvironment and exhibit high CTLA-4 expression