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Metabolomics profiling of mouse plasma, MRS medium, and bacterial supernatants Ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) analysis Four volumes of 80% methanol in water were added to 20 L of mouse plasma, bacterial culture supernatants, or MRS media

The cold dissolving is slower but gentler

Halagali P, Nayak D, Tippavajhala VK, Rathnanand M, Biswas D, Sharma H

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At this point, the embryo likely becomes necrotic and is simply resorbed by the mother (Bondareva et al., 2007)