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To ensure that the compounds identified in our parent and pDC-based assays were specific inhibitors of the interaction between STX7 and Munc13-4 but did not interfere with vesicle docking and exocytosis by inhibiting RabsMunc13-4 complexes, ENDO compounds were counterscreened in TR-FRET assays using lysates expressing FlagMunc13-4 and EGFPRab27a or EGFPRab11

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Palma E, Mendes F, Morais GR, Rodrigues I, Santos IC, Campello MPC, Raposinho P, Correia I, Gama S, Belo D, Alves V, Abrunhosa AJ, Santos I, Paulo A (2017) Biophysical characterization and antineoplastic activity of new bis(thiosemicarbazonato) Cu(II) complexes