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Abstract The deposition of ectopic lipids and resulting insulin resistance (IR) are strongly linked to a high risk of many chronic metabolic diseases, such as type 2 diabetes mellitus (T2D) and metabolic dysfunctionassociated steatotic liver disease (MASLD)

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Blood Cancer 61 , 982986 (2014)

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Membranes were incubated with primary antibodies diluted in 5% milk 1X TBST overnight at 4C: anti-Actin (1:3000) (Sigma, A2066), anti-NRF2 (1:1000) (Cell Signaling Technology #12721) and anti-GSR (1:1000) (ThermoFisher, PA5-29945), followed by washing and incubation with HRP-conjugated anti-rabbit IgG (1:2000) (CST#7074) in 5% milk 1X TBST (22C, 1 h)